Coding
EforRed

Part:BBa_K3892006:Design

Designed by: iGEM21_Linkoping_Sweden   Group: iGEM21_Linkoping_Sweden   (2021-10-17)


J23110-G10 leader-EforRed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 75
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added a BamHI site to the start of the protein to make it easier to change the promotor/RBS. Only a glycine is added which did not impact folding. Also enables swapping of the downstream protein.


Source

EforRed (Echinopora forskaliana)

References